Integrating database information in microarray expression analyses: Application to melanoma cell lines profiled in the NCI60 data set.

نویسندگان

  • Hong Dang
  • Tin Le
  • Toby Segaran
  • Joshua Levy
چکیده

A series of successful microarray experiments typically results in a data matrix containing a list of a few thousand genes and their measured expression values across various conditions tested. The understanding of why some genes are clustered in their expression pattern can be greatly facilitated by gathering all prior knowledge about all the genes in a given cluster. Here we demonstrate a strategy using the NCI60 data set (Scherf U, Ross DT,Waltham M, et al., Nature Genet 2000;24(3):236-244) on a cluster of genes highly expressed in the melanoma cell lines. By traversing public databases using cross-references between the databases,we show that the Gene Ontology (GO) classifications of some of the genes are consistent with their products' involvement in melanin biosynthesis.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

MACE: mutation-oriented profiling of chemical response and gene expression in cancers

SUMMARY The mutational status of specific cancer lineages can affect the sensitivity to or resistance against cancer drugs. The MACE database provides web-based interactive tools for interpreting large chemical screening and gene expression datasets of cancer cell lines in terms of mutation and lineage categories. GI50 data of chemicals against individual NCI60 cell lines were normalized and or...

متن کامل

Using the Protein-protein Interaction Network to Identifying the Biomarkers in Evolution of the Oocyte

Background Oocyte maturity includes nuclear and cytoplasmic maturity, both of which are important for embryo fertilization. The development of oocyte is not limited to the period of follicular growth, and starts from the embryonic period and continues throughout life. In this study, for the purpose of evaluating the effect of the FSH hormone on the expression of genes, GEO access codes for this...

متن کامل

Global gene expression analysis using microarray to study differential vulnerability to neurodegeneration

Neurodegenerative disorders such as Parkinson’s disease, motor neuron disease and Alzheimer’s disease is characterized by loss of specific cells within certain regions of the brain. One of the most compelling questions is to determine why specific cell populations are vulnerable to neurodegeneration. We addressed this question by studying global gene expression changes using an animal model of ...

متن کامل

Global gene expression analysis using microarray to study differential vulnerability to neurodegeneration

Neurodegenerative disorders such as Parkinson’s disease, motor neuron disease and Alzheimer’s disease is characterized by loss of specific cells within certain regions of the brain. One of the most compelling questions is to determine why specific cell populations are vulnerable to neurodegeneration. We addressed this question by studying global gene expression changes using an animal model of ...

متن کامل

CYTOKINE AND GROWTH FACTOR MODULATION OF CELL CYCLE EVENTS IN HUMAN MELANOMA CELL LINES

Cytokines influence cell cycle events, which in some but not all instances can be associated with melanoma progression. Analysis of the G0/G 1 and S phase fractions of the cell cycle was used to assay the proliferative or inhibitory activity of cytokines against ten human melanoma cell lines, including pairs of cell lines derived from primary and metastatic tissue of individual patients. Cy...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of biomolecular techniques : JBT

دوره 13 4  شماره 

صفحات  -

تاریخ انتشار 2002